Bhalla Lab

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C. elegans meiotic nuclei undergoing synaptonemal complex disassembly in late meiotic prophase. DNA is blue, synaptonemal complex component, SYP-1, is red and recombination protein, ZHP-3, is green. 

C. elegans meiotic nuclei undergoing synaptonemal complex disassembly in late meiotic prophase. DNA is blue, synaptonemal complex component, SYP-1, is red and recombination protein, ZHP-3, is green. 

Lab Resources:

Bhalla Antibody Database

Protocols:

Worm Viability Assay

Worm Viability Spreadsheet

Worm Genomic Prep

Trizol Extraction of RNA

Ethanol Precipitation

Co-immunoprecipitation

Worm Lysate Prep & Western Blot Analysis

Western Blot of Germline Proteins

HeLa Cell Immunostaining

10Beta Competent Cell Prep

Immunofluorescence

PCR from Worm Lysate

PCR from Genomic Prep

Live Imaging/Embryo Microscopy

CRISPR

coCRISPR RNP Mix Assembly

Bacterial Transformation (10Beta)

Feeding RNAi

Scoring Apoptosis

Freezing Worms

Bleaching Worms

 

 

 

 

 

The Bhalla Lab is located in the Department of Molecular, Cell and Developmental Biology at the University of California, Santa Cruz. All material is copyright to Needhi Bhalla, unless otherwise indicated.