C. elegans meiotic nuclei undergoing synaptonemal complex disassembly in late meiotic prophase. DNA is blue, synaptonemal complex component, SYP-1, is red and recombination protein, ZHP-3, is green.
Lab Resources:
Bhalla Antibody Database
Protocols:
Immunofluorescence
PCR from Worm Lysate
PCR from Genomic Prep
Live Imaging/Embryo Microscopy
coCRISPR RNP Mix Assembly
Bacterial Transformation (10Beta)
Feeding RNAi
Scoring Apoptosis
Freezing Worms
Bleaching Worms
Worm Viability Assay
Worm Viability Spreadsheet
Worm Genomic Prep
Trizol Extraction of RNA
Ethanol Precipitation
Co-immunoprecipitation
Worm Lysate Prep & Western Blot Analysis
HeLa Cell Immunostaining
10Beta Competent Cell Prep